EC67844
(Plasmid
#209514)
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Purpose(Empty Backbone) Cloning in complete guide expression cassettes, with Cas9
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeCRISPR
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Selectable markersRFP & hygromycin for plant
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Wheat GoldenGate binary vector with Cas9
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EC67844 was a gift from Wendy Harwood & Cristobal Uauy (Addgene plasmid # 209514 ; http://n2t.net/addgene:209514 ; RRID:Addgene_209514) -
For your References section:
An Optimised CRISPR Cas9 and Cas12a Toolkit for Barley and Wheat. Lawrenson T, Clarke M, Kirby R, Forner M, Steuernagel B, Harwood W. Research Square, 12 March 2024, PREPRINT 10.21203/rs.3.rs-4024107/v1