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Addgene

pGL2-AN
(Plasmid #20950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGl2-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5533
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2F-1 promoter, genomic fragment
  • Alt name
    E2F1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    273
  • Mutation
    wt human E2F-1 promoter sequence
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site Nco I (destroyed during cloning)
  • 5′ sequencing primer GLprimer1
  • 3′ sequencing primer GLprimer2
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-AN was a gift from William Kaelin (Addgene plasmid # 20950 ; http://n2t.net/addgene:20950 ; RRID:Addgene_20950)

  • For your References section:

    Transcription of the E2F-1 gene is rendered cell cycle dependent by E2F DNA-binding sites within its promoter. Neuman E, Flemington EK, Sellers WR, Kaelin WG. Mol Cell Biol. 1994 Oct . 14(10):6607-15. PubMed 7935380
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