pcDNA3.1/opto-a1AR-EYFP
(Plasmid #20947)

• Purpose: Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling
• Depositing Lab: Karl Deisseroth
• Publication: Airan et al Nature. 2009 Mar 18. ():.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: opto-a1AR
• Species: synthetic
• Insert Size (bp): 2208

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see http://www.optogenetics.org

for additional information.