EC70206
(Plasmid
#209467)
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PurposeCloning protospacers for Cas9 editing
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209467 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeCRISPR
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Selectable markersLacZ
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9 sgRNA
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SpeciesSteptococcus pyogenes
- Promoter TaU6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GoldeGate level 1 position 5
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EC70206 was a gift from Wendy Harwood & Cristobal Uauy (Addgene plasmid # 209467 ; http://n2t.net/addgene:209467 ; RRID:Addgene_209467) -
For your References section:
An Optimised CRISPR Cas9 and Cas12a Toolkit for Barley and Wheat. Lawrenson T, Clarke M, Kirby R, Forner M, Steuernagel B, Harwood W. Research Square, 12 March 2024, PREPRINT 10.21203/rs.3.rs-4024107/v1