pLenti-CaMKIIa-eNpHR-EYFP-WPRE
(Plasmid #20946)

• Purpose: 3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition
• Depositing Lab: Karl Deisseroth
• Publication: Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Backbone

• Vector backbone: pLenti-CaMKIIa
• Backbone size w/o insert (bp): 9277
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: halorhodopsin
• Alt name: HR
• Alt name: NpHR
• Alt name: eNpHR
• Species: N. pharaonis
• Insert Size (bp): 1647
• Promoter: CaMKIIa
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry