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pLenti-CaMKIIa-eNpHR-EYFP-WPRE
(Plasmid #20946)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-CaMKIIa
  • Backbone size w/o insert (bp) 9277
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    halorhodopsin
  • Alt name
    HR
  • Alt name
    NpHR
  • Alt name
    eNpHR
  • Species
    N. pharaonis
  • Insert Size (bp)
    1647
  • Promoter CaMKIIa
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CaMKIIa-eNpHR-EYFP-WPRE was a gift from Karl Deisseroth (Addgene plasmid # 20946 ; http://n2t.net/addgene:20946 ; RRID:Addgene_20946)

  • For your References section:

    Multimodal fast optical interrogation of neural circuitry. Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, Bamberg E, Nagel G, Gottschalk A, Deisseroth K. Nature. 2007 Apr 5. 446(7136):633-9. 10.1038/nature05744 PubMed 17410168
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