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Addgene

EC70197
(Plasmid #209458)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    CRISPR
  • Selectable markers
    LacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9 sgRNA
  • Species
    Steptococcus pyogenes
  • Promoter TaU3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GoldeGate level 1 position 3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EC70197 was a gift from Wendy Harwood & Cristobal Uauy (Addgene plasmid # 209458 ; http://n2t.net/addgene:209458 ; RRID:Addgene_209458)

  • For your References section:

    An Optimised CRISPR Cas9 and Cas12a Toolkit for Barley and Wheat. Lawrenson T, Clarke M, Kirby R, Forner M, Steuernagel B, Harwood W. Research Square, 12 March 2024, PREPRINT 10.21203/rs.3.rs-4024107/v1
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