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Addgene

pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: AgeI and EcoRI (single digests)

We expected the following band sizes for this digest: ~10kb for each. We see the expected pattern.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with EcoRI.
This should linearize the plasmid, we see the expected pattern.