pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzymes: AgeI and EcoRI (single digests)
We expected the following band sizes for this digest: ~10kb for each. We see the expected pattern.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with EcoRI.
This should linearize the plasmid, we see the expected pattern.