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pCRISPR-Cas3
(Plasmid #209427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGM1190
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Cultivation of streptomycetes at 30 C. Temperature sensitive pSG5 replicon, only stable below 37 C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
  • Alt name
    CASCADE-Cas3
  • Species
    Synthetic; Pseudomonas aeruginosa
  • Insert Size (bp)
    5601
  • Promoter PtipA; crRNA under control of PermE*

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.05.09.539971 for bioRxiv preprint.

The used type I-C CRISPR system was first described by:
Csörgő, B., León, L.M., Chau-Ly, I.J. et al. A compact Cascade–Cas3 system for targeted genome engineering. Nat Methods 17, 1183–1190 (2020). https://doi.org/10.1038/s41592-020-00980-w

Plasmid contains an IS prokaryotic transposable element that does not affect plasmid function.

The sequences used in pCRISPR-Cas3 were obtained via gene synthesis following codon optimization for S. coelicolor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPR-Cas3 was a gift from Tilmann Weber (Addgene plasmid # 209427 ; http://n2t.net/addgene:209427 ; RRID:Addgene_209427)

  • For your References section:

    CASCADE-Cas3 Enables Highly Efficient Genome Engineering in Streptomyces Species. Whitford CM, Gockel P, Faurdal D, Gren T, Sigrist R, Weber T. bioRxiv 2023.05.09.539971 10.1101/2023.05.09.539971
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