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pET29b_mNeonGreen
(Plasmid #209425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5235
  • Total vector size (bp) 5978
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) strain for protein expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mNeonGreen
  • Insert Size (bp)
    744
  • GenBank ID
    KC295282.1 AGG56535
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b_mNeonGreen was a gift from Magnus Rueping (Addgene plasmid # 209425 ; http://n2t.net/addgene:209425 ; RRID:Addgene_209425)

  • For your References section:

    Nanobody-Based Lateral Flow Immunoassay for Rapid Antigen Detection of SARS-CoV-2 and MERS-CoV Proteins. Peng Y, Huang Y, Kiessling F, Renn D, Rueping M. ACS Synth Biol. 2025 Jan 9. doi: 10.1021/acssynbio.4c00592. 10.1021/acssynbio.4c00592 PubMed 39786915
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