pLenti-EF1a-hChR2(H134R)-EYFP-WPRE
(Plasmid #20942)

• Purpose: 3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation
• Depositing Lab: Karl Deisseroth
• Publication: Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Backbone

• Vector backbone: pLenti-EF1a
• Backbone size w/o insert (bp): 9000
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: channelrhodopsin-2
• Alt name: ChR2
• Alt name: cop4
• Alt name: channelopsin-2
• Species: C. reinhardtii
• Insert Size (bp): 1662
• Mutation: H134R mutation in ChR2
• Promoter: EF1alpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see http://www.optogenetics.org

for additional information.

The H134R mutation present in ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence