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pET29_anti-MERS-Nanobody-VHH-83
(Plasmid #209418)

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Item Catalog # Description Quantity Price (USD)
Plasmid 209418 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET29
  • Backbone size w/o insert (bp) 5235
  • Total vector size (bp) 5627
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use SHuffle T7 strain for protein expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    anti-mers-nanobody-vhh-83
  • Insert Size (bp)
    393
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Raj and Okba et al. 2018 PMID 30101189

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29_anti-MERS-Nanobody-VHH-83 was a gift from Magnus Rueping (Addgene plasmid # 209418 ; http://n2t.net/addgene:209418 ; RRID:Addgene_209418)

  • For your References section:

    Nanobody-Based Lateral Flow Immunoassay for Rapid Antigen Detection of SARS-CoV-2 and MERS-CoV Proteins. Peng Y, Huang Y, Kiessling F, Renn D, Rueping M. ACS Synth Biol. 2025 Jan 9. doi: 10.1021/acssynbio.4c00592. 10.1021/acssynbio.4c00592 PubMed 39786915
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