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pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet
(Plasmid #209411)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209411 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4TO-IRES-mScarlet
  • Backbone size w/o insert (bp) 6465
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SCN8A Variant 3, stabilized
  • Alt name
    Nav1.6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6165
  • Mutation
    Modifed human HSPA5 intron 4 inserted after bp2370 of the SCN8A ORF. IVS intron inserted after bp4370 of the SCN8A ORF. Silent codon changes at bp4208A>G and bp4211T>C of the SCN8A ORF.
  • Entrez Gene
    SCN8A (a.k.a. BFIS5, CERIII, CIAT, DEE13, EIEE13, MED, MYOCL2, NaCh6, Nav1.6, PN4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer V1-5
  • 3′ sequencing primer pcDNA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The SCN8A channel cDNA has been altered to be stably maintained in standard E.coli strains at high copy under standard growth conditions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet was a gift from Al George (Addgene plasmid # 209411 ; http://n2t.net/addgene:209411 ; RRID:Addgene_209411)

  • For your References section:

    Molecular and cellular context influences SCN8A variant function. Vanoye CG, Abramova TV, DeKeyser JM, Ghabra NF, Oudin MJ, Burge CB, Helbig I, Thompson CH, George AL Jr. JCI Insight. 2024 May 21;9(12):e177530. doi: 10.1172/jci.insight.177530. 10.1172/jci.insight.177530 PubMed 38771640
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