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pIR-CMV-SCN2A-Variant-4-IRES-mScarlet
(Plasmid #209410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIR-CMV-IRES-mScarlet
  • Backbone size w/o insert (bp) 6151
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SCN2A Variant 4, stabilized
  • Alt name
    Nav1.2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6151
  • Mutation
    IVS intron inserted after bp 4551 of the SCN2A ORF.
  • GenBank ID
    NM_001371246.1
  • Entrez Gene
    SCN2A (a.k.a. BFIC3, BFIS3, BFNIS, DEE11, EA9, EIEE11, HBA, HBSCI, HBSCII, NAC2, Na(v)1.2, Nav1.2, SCN2A1, SCN2A2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer V1-5
  • 3′ sequencing primer Sv40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The SCN2A channel cDNA has been altered to be stably maintained in standard E.coli strains at high copy under standard growth conditions.

Please visit https://www.biorxiv.org/content/10.1101/2023.02.23.529757v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIR-CMV-SCN2A-Variant-4-IRES-mScarlet was a gift from Al George (Addgene plasmid # 209410 ; http://n2t.net/addgene:209410 ; RRID:Addgene_209410)

  • For your References section:

    Epilepsy-associated SCN2A (Na (V) 1.2) Variants Exhibit Diverse and Complex Functional Properties. Thompson CH, Potet F, Abramova TV, DeKeyser JM, Ghabra NF, Vanoye CG, Millichap J, George AL Jr. bioRxiv. 2023 Feb 23:2023.02.23.529757. doi: 10.1101/2023.02.23.529757. Preprint. 10.1101/2023.02.23.529757 PubMed 36865317
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