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Addgene

pGG-NES-TurboID-GFP
(Plasmid #209401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LII alpha F1-2
  • Backbone manufacturer
    Parniske lab
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-3XHA-TurboID
  • Species
    (TurboID) BirA from E. coli;NES is from HEAT STABLE INHIBITOR (PKI) .
  • Mutation
    TurboID gene sequence C249A
  • Promoter Cauliflower mosaic virus (CaMV) 35S promoter
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGG-NES-TurboID-GFP was a gift from Rosa Lozano-Durán (Addgene plasmid # 209401 ; http://n2t.net/addgene:209401 ; RRID:Addgene_209401)

  • For your References section:

    The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana. Tan H, Zhou Y, Dinius E, Lozano-Duran R. J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610. 10.1111/jipb.13610 PubMed 38294100
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