pGG-GTS-TurboID
(Plasmid #209397)

• Purpose: Transiently expressing TurboID fused with a Golgi targeting sequence (GTS) in planta
• Depositing Lab: Rosa Lozano-Durán
• Publication: Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610.

Backbone

• Vector backbone: LII alpha F1-2
• Backbone manufacturer: Parniske lab
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GTS-3XHA-TurboID
• Species: (TurboID) BirA from E. coli. GTS is from ALPHA-MANNOSIDASE 3 (AT1G30000) .
• Mutation: TurboID gene sequence C249A
• Promoter: Cauliflower mosaic virus (CaMV) 35S promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: 35S promoter
• 3′ sequencing primer: ACCGCGCGCGATAATTTAT

Resource Information

• Supplemental Documents:
  • pGG-GTS-TurboID.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.