pGG-NES-TurboID
(Plasmid
#209393)
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PurposeTransiently expressing TurboID fused with a nuclear export signal (NES) in planta
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209393 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLII alpha F1-2
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Backbone manufacturerParniske lab
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNES-3XHA-TurboID
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Species(TurboID) BirA from E. coli;NES is from HEAT STABLE INHIBITOR (PKI) .
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MutationTurboID gene sequence C249A
- Promoter Cauliflower mosaic virus (CaMV) 35S promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer 35S promoter
- 3′ sequencing primer ACCGCGCGCGATAATTTAT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGG-NES-TurboID was a gift from Rosa Lozano-Durán (Addgene plasmid # 209393 ; http://n2t.net/addgene:209393 ; RRID:Addgene_209393) -
For your References section:
The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana. Tan H, Zhou Y, Dinius E, Lozano-Duran R. J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610. 10.1111/jipb.13610 PubMed 38294100