pGW-GFP-GOI-TurboID
(Plasmid
#209382)
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Purpose(Empty Backbone) Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209382 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGWB506
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Backbone manufacturerhttp://shimane-u.org/nakagawa/gbv.htm
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationTurboID gene sequence C249A
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
3XHA-TurboID sequence was amplified from 3xHA-TurboID-NLS_pCDNA3 (Addgene#107171). The 249th nucleotide cytosine (C) of TurboID was changed to adenine (A) without affecting the TurboID protein sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGW-GFP-GOI-TurboID was a gift from Rosa Lozano-Durán (Addgene plasmid # 209382 ; http://n2t.net/addgene:209382 ; RRID:Addgene_209382) -
For your References section:
The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana. Tan H, Zhou Y, Dinius E, Lozano-Duran R. J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610. 10.1111/jipb.13610 PubMed 38294100