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Addgene

pcDNA3-HA2-PARC
(Plasmid #20937)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20937 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5512
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53-associated parkin-like cytoplasmic protein
  • Alt name
    PARC
  • Alt name
    CUL9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5263
  • Mutation
    S1049N, C1076R, R2389L
  • GenBank ID
    NM_015089
  • Entrez Gene
    CUL9 (a.k.a. H7AP1, PARC)
  • Tag / Fusion Protein
    • HA2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA2-PARC was a gift from Yue Xiong (Addgene plasmid # 20937 ; http://n2t.net/addgene:20937 ; RRID:Addgene_20937)
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