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Addgene

pcDNA3-myc3-L11
(Plasmid #20936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5437
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ribosomal protein L11
  • Alt name
    RPL11
  • Alt name
    L11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • GenBank ID
    NM_000975
  • Entrez Gene
    RPL11 (a.k.a. DBA7, GIG34, L11, uL5)
  • Tag / Fusion Protein
    • myc3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl2/EcoRI (destroyed during cloning)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc3-L11 was a gift from Yue Xiong (Addgene plasmid # 20936 ; http://n2t.net/addgene:20936 ; RRID:Addgene_20936)

  • For your References section:

    Ribosomal protein L11 negatively regulates oncoprotein MDM2 and mediates a p53-dependent ribosomal-stress checkpoint pathway. Zhang Y, Wolf GW, Bhat K, Jin A, Allio T, Burkhart WA, Xiong Y. Mol Cell Biol. 2003 Dec . 23(23):8902-12. 10.1128/MCB.23.23.8902-8912.2003 PubMed 14612427
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