pCMV-myc3-HDM2
(Plasmid #20935)

• Depositing Lab: Yue Xiong
• Publication: Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12.

Backbone

• Vector backbone: pCMV
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3800
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mdm2 p53 binding protein homolog
• Alt name: HDM2 (MYO-32 transcript variant)
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• GenBank ID: AFM80540.1
• Entrez Gene: MDM2 (a.k.a. ACTFS, HDMX, LSKB, hdm2)
• Tag / Fusion Protein:
  • myc3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry