EP003: CAG::GEM7-HaloTag
(Plasmid #209303)

• Purpose: Expression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporation
• Depositing Lab: Adam Cohen
• Publication: Expression Plasmids for Production of Particles Decorated with HaloTag (unpublished)

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5802
• Total vector size (bp): 7572
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GEM7
• Alt name: Family 1 encapsulin nanocompartment shell protein
• Species: R. rubrum
• Insert Size (bp): 1770
• GenBank ID: WP_011388729.1
• Tag / Fusion Protein:
  • HaloTag (C terminal on insert)

Resource Information

• Supplemental Documents:
  • CAG174_CAG-GEM7-HaloTag.gb
• A portion of this plasmid was derived from a plasmid made by: Julia Mahamid (European Molecular Biology Laboratory)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry