pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato
(Plasmid #209298)

• Purpose: A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.
• Depositing Lab: Birgitt Schuele
• Publication: Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 10.1038/s41598-023-45078-3.

Backbone

• Vector backbone: plenti
• Total vector size (bp): 14054
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Tetracycline

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: dCas9
• Alt name: SadCas9
• Promoter: tetracycline-inducible promoter (TRE3G)

Cloning Information for Gene/Insert 1

• Cloning method: TOPO Cloning
• 5′ sequencing primer: accgcgggcccgggggatccATGGCCCCAAAGAAGAAGCG
• 3′ sequencing primer: TTGATGCCGCTCAGCTCG

Gene/Insert 2

• Gene/Insert name: tdTomato

Resource Information

• A portion of this plasmid was derived from a plasmid made by: A gift from Professor Stanley Qi (Stanford) where zeocin resistance gene (zeo) was replaced by a fluorescent marker, tdTomato

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.01.24.525105v1 for bioRxiv preprint.