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pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII
(Plasmid #209292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET45b(+)
  • Backbone manufacturer
    Millipore Sigma
  • Backbone size w/o insert (bp) 6640
  • Total vector size (bp) 13852
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    R2
  • Alt name
    R2Bm
  • Alt name
    Reverse transcriptase-like protein
  • Alt name
    Bombyx mori rDNA insertion element R2 (typeII), complete cds
  • Species
    Bombyx mori
  • Insert Size (bp)
    7212
  • GenBank ID
    M16558.1
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • MBP (N terminal on backbone)
    • bdSUMO (N terminal on backbone)
    • SpCas9(H840A) (C terminal on insert)
    • StrepII (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer Whole plasmid sequencing
  • 3′ sequencing primer Whole plasmid sequencing
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII was a gift from Feng Zhang (Addgene plasmid # 209292 ; http://n2t.net/addgene:209292 ; RRID:Addgene_209292)

  • For your References section:

    Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription. Wilkinson ME, Frangieh CJ, Macrae RK, Zhang F. Science. 2023 Apr 21;380(6642):301-308. doi: 10.1126/science.adg7883. Epub 2023 Apr 6. 10.1126/science.adg7883 PubMed 37023171
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