pMW37: pRS426-R2Bm RNA
(Plasmid #209291)

• Purpose: Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences
• Depositing Lab: Feng Zhang
• Publication: Wilkinson et al Science. 2023 Apr 21;380(6642):301-308. doi: 10.1126/science.adg7883. Epub 2023 Apr 6.

Backbone

• Vector backbone: pRS426
• Backbone size w/o insert (bp): 6795
• Total vector size (bp): 8221
• Modifications to backbone: 3xMS2 tag added
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: R2Bm 5'UTR and 3'UTR
• Alt name: R2Bm
• Alt name: Bombyx mori rDNA insertion element R2 (typeII)
• Species: Bombyx mori
• Insert Size (bp): 1426
• GenBank ID: M16558.1
• Promoter: GAL1 GAPDH

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Whole plasmid sequencing
• 3′ sequencing primer: Whole plasmid sequencing

Resource Information

• Supplemental Documents:
  • pMW37_pRS426-R2Bm-RNA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry