pMW41: pET-His-MBP-SUMO-R2Bm-SII
(Plasmid #209290)

• Purpose: Expresses the R2Bm ORF in E. coli
• Depositing Lab: Feng Zhang
• Publication: Wilkinson et al Science. 2023 Apr 21;380(6642):301-308. doi: 10.1126/science.adg7883. Epub 2023 Apr 6.

Backbone

• Vector backbone: pET45b(+)
• Backbone manufacturer: Millipore Sigma
• Backbone size w/o insert (bp): 6640
• Total vector size (bp): 9652
• Modifications to backbone: add 14xHis-MBP-bdSUMO and TwinStrepII tags
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: R2
• Alt name: R2Bm
• Alt name: Reverse transcriptase-like protein
• Alt name: Bombyx mori rDNA insertion element R2 (typeII), complete cds
• Species: Bombyx mori
• Insert Size (bp): 3012
• GenBank ID: M16558.1
• Promoter: T7
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • MBP (N terminal on backbone)
  • bdSUMO (N terminal on backbone)
  • StrepII (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Whole plasmid sequencing
• 3′ sequencing primer: Whole plasmid sequencing

Resource Information

• Supplemental Documents:
  • pMW41_pET-His-MBP-SUMO-R2Bm-SII.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry