mini-donor - NKX2.5-nEGFP
(Plasmid
#209287)
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Purposethis mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC-GW-Amp
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Backbone manufacturerAzenta
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Vector typeknockin donor vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP-nls
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mini-donor - NKX2.5-nEGFP was a gift from Xiaojun Lian (Addgene plasmid # 209287 ; http://n2t.net/addgene:209287 ; RRID:Addgene_209287) -
For your References section:
MAGIK: A rapid and efficient method to create lineage-specific reporters in human pluripotent stem cells. Haideri T, Lin J, Bao X, Lian XL. Stem Cell Reports, 2024 10.1016/j.stemcr.2024.03.005