pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f
(Plasmid
#209283)
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PurposeExpresses Voltron1 and jGCaMP7f in the zebrafish heart
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 209283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonezACNE20_myl7
- Backbone size w/o insert (bp) 7820
- Total vector size (bp) 10789
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVoltron1_P2A_jGCaMP7f
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SpeciesSynthetic
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Insert Size (bp)2970
- Promoter zACNE20_myl7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cctgctggaatctgagca (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f was a gift from Adam Cohen (Addgene plasmid # 209283 ; http://n2t.net/addgene:209283 ; RRID:Addgene_209283) -
For your References section:
A bioelectrical phase transition patterns the first vertebrate heartbeats. Jia BZ, Qi Y, Wong-Campos JD, Megason SG, Cohen AE. Nature. 2023 Oct;622(7981):149-155. doi: 10.1038/s41586-023-06561-z. Epub 2023 Sep 27. 10.1038/s41586-023-06561-z PubMed 37758945
