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pBJ016_MTB_CoChR_KGC_eGFP_ER2
(Plasmid #209277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMTB
  • Backbone size w/o insert (bp) 6329
  • Total vector size (bp) 8054
  • Vector type
    Zebrafish expression, in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CoChR_KGC_eGFP_ER2
  • Species
    Synthetic
  • Insert Size (bp)
    1725
  • Promoter actb2

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atggtcacatgcttgctg
  • 3′ sequencing primer gcaatagcatcacaaatttc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBJ016_MTB_CoChR_KGC_eGFP_ER2 was a gift from Adam Cohen (Addgene plasmid # 209277 ; http://n2t.net/addgene:209277 ; RRID:Addgene_209277)

  • For your References section:

    A bioelectrical phase transition patterns the first vertebrate heartbeats. Jia BZ, Qi Y, Wong-Campos JD, Megason SG, Cohen AE. Nature. 2023 Oct;622(7981):149-155. doi: 10.1038/s41586-023-06561-z. Epub 2023 Sep 27. 10.1038/s41586-023-06561-z PubMed 37758945
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