pEP4 E02S ET2K Notes
Addgene diagnostic digest for 20927
This gel depicts the NEB 1kb ladder and a digestion of the plasmid 20927 with the following enzyme: EcoRI-HF. The expected pattern of bands was observed.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid pEP4 E02S ET2K with the following enzymes: EcoRV-HF, NotI-HF, and ScaI-HF.
Expected Bands
EcoRV-HF:17.5 kb
EcoRv-HF/ NotI-HF:14.4 kb + 3.1 kb
EcoRV-HF/ ScaI-HF:12.7 kb + 4.8 kb
We see the expected bands.