pMx/ATP10D(E215Q)-HA
(Plasmid
#209260)
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PurposeMammalian expression of ATP10D mutant E215Q
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMx
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATP10D
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SpeciesH. sapiens (human)
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MutationE215Q
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Entrez GeneATP10D (a.k.a. ATPVD)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMx/ATP10D(E215Q)-HA was a gift from Hye-Won Shin (Addgene plasmid # 209260 ; http://n2t.net/addgene:209260 ; RRID:Addgene_209260) -
For your References section:
Phospholipid Flippase ATP10A Translocates Phosphatidylcholine and Is Involved in Plasma Membrane Dynamics. Naito T, Takatsu H, Miyano R, Takada N, Nakayama K, Shin HW. J Biol Chem. 2015 Jun 12;290(24):15004-17. doi: 10.1074/jbc.M115.655191. Epub 2015 May 6. 10.1074/jbc.M115.655191 PubMed 25947375