pCEP4-M2L
(Plasmid #20926)

• Purpose: used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28
• Depositing Lab: James Thomson
• Publication: Yu et al Science. 2009 Mar 26.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10200
• Vector type: Episomal/EBNA
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: "Extremely" low copy number
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: MYC
• Alt name: bHLEHE39
• Alt name: c-Myc
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• GenBank ID: NM_002467.4
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: IRES-R2 (gacggcaatatggtggaaa)

Gene/Insert 2

• Gene/Insert name: LIN28
• Alt name: CSDD1
• Alt name: FLJ12457
• Alt name: LIN-28
• Species: H. sapiens (human)
• Insert Size (bp): 600
• GenBank ID: NM_024674.4
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
• Promoter: IRES

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: IRES-F
• 3′ sequencing primer: EBV_rev

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Myc and Lin28 are coexpressed under a strong CMV promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells.

These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul.