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Addgene

pCAG/ATP11B(E180Q)-HA
(Plasmid #209250)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP11B
  • Species
    H. sapiens (human)
  • Mutation
    E180Q
  • Entrez Gene
    ATP11B (a.k.a. ATPIF, ATPIR)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid requires co-transfection with Addgene Plasmid 209223 to ensure the cellular localization of the encoded protein (Takatsu et al., 2011, JBC).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG/ATP11B(E180Q)-HA was a gift from Hye-Won Shin (Addgene plasmid # 209250 ; http://n2t.net/addgene:209250 ; RRID:Addgene_209250)

  • For your References section:

    ATPase reaction cycle of P4-ATPases affects their transport from the endoplasmic reticulum. Tone T, Nakayama K, Takatsu H, Shin HW. FEBS Lett. 2020 Feb;594(3):412-423. doi: 10.1002/1873-3468.13629. Epub 2019 Oct 14. 10.1002/1873-3468.13629 PubMed 31571211
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