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Addgene

pCAG/ATP8B4(E170Q)-HA
(Plasmid #209243)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209243 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP8B4
  • Species
    H. sapiens (human)
  • Mutation
    E170Q
  • Entrez Gene
    ATP8B4 (a.k.a. ATPIM, FLJ25418)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid requires co-transfection with Addgene Plasmid 209223 to ensure the cellular localization of the encoded protein (Takatsu et al., 2011, JBC).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG/ATP8B4(E170Q)-HA was a gift from Hye-Won Shin (Addgene plasmid # 209243 ; http://n2t.net/addgene:209243 ; RRID:Addgene_209243)

  • For your References section:

    Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane. Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin HW. J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 10.1074/jbc.M114.593012. Epub 2014 Oct 14. 10.1074/jbc.M114.593012 PubMed 25315773
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