pMx/ATP8B4-HA
(Plasmid
#209229)
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PurposeMammalian expression of ATP8B4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMx
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATP8B4
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SpeciesH. sapiens (human)
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Entrez GeneATP8B4 (a.k.a. ATPIM, FLJ25418)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMx/ATP8B4-HA was a gift from Hye-Won Shin (Addgene plasmid # 209229 ; http://n2t.net/addgene:209229 ; RRID:Addgene_209229) -
For your References section:
Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane. Takatsu H, Tanaka G, Segawa K, Suzuki J, Nagata S, Nakayama K, Shin HW. J Biol Chem. 2014 Nov 28;289(48):33543-56. doi: 10.1074/jbc.M114.593012. Epub 2014 Oct 14. 10.1074/jbc.M114.593012 PubMed 25315773