pAAV-CAG-FLEX-Chrimson-TdTomato
(Plasmid
#209202)
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PurposeCre-dependent expression of mCherry-tagged Chrimson; Optogenetics
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209202 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChrimson
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Speciesother
- Promoter CAG
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Tag
/ Fusion Protein
- tdTomato (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CAG-FLEX-Chrimson-TdTomato was a gift from Larry Zweifel (Addgene plasmid # 209202 ; http://n2t.net/addgene:209202 ; RRID:Addgene_209202) -
For your References section:
Anatomic resolution of neurotransmitter-specific projections to the VTA reveals diversity of GABAergic inputs. Soden ME, Chung AS, Cuevas B, Resnick JM, Awatramani R, Zweifel LS. Nat Neurosci. 2020 Aug;23(8):968-980. doi: 10.1038/s41593-020-0657-z. Epub 2020 Jun 15. 10.1038/s41593-020-0657-z PubMed 32541962