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Addgene

pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)
(Plasmid #209198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-FLEX-SaCas9-U6-sgRNA
  • Backbone manufacturer
    Larry Zweifel (Addgene plasmid # 124844)
  • Vector type
    Mouse Targeting, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Th
  • gRNA/shRNA sequence
    GGACGGCGGCAGAGTCTCATC
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Th
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-FLEX-SaCas9-U6-sgTh(2) was a gift from Larry Zweifel (Addgene plasmid # 209198 ; http://n2t.net/addgene:209198 ; RRID:Addgene_209198)

  • For your References section:

    Periaqueductal gray/dorsal raphe dopamine neurons contribute to sex differences in pain-related behaviors. Yu W, Pati D, Pina MM, Schmidt KT, Boyt KM, Hunker AC, Zweifel LS, McElligott ZA, Kash TL. Neuron. 2021 Apr 21;109(8):1365-1380.e5. doi: 10.1016/j.neuron.2021.03.001. Epub 2021 Mar 18. 10.1016/j.neuron.2021.03.001 PubMed 33740416
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