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Addgene

pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP
(Plasmid #209194)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209194 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kcnq3
  • Species
    H. sapiens (human)
  • Mutation
    R230 to C230
  • Entrez Gene
    KCNQ3 (a.k.a. BFNC2, EBN2, KV7.3)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP was a gift from Larry Zweifel (Addgene plasmid # 209194 ; http://n2t.net/addgene:209194 ; RRID:Addgene_209194)

  • For your References section:

    Autism-associated mutations in K(V)7 channels induce gating pore current. Gamal El-Din TM, Lantin T, Tschumi CW, Juarez B, Quinlan M, Hayano JH, Li J, Zweifel LS, Catterall WA. Proc Natl Acad Sci U S A. 2021 Nov 9;118(45):e2112666118. doi: 10.1073/pnas.2112666118. 10.1073/pnas.2112666118 PubMed 34728568
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