pAAV-FLEX-HM4Di-YFP
(Plasmid #209193)

• Purpose: Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics
• Depositing Lab: Larry Zweifel
• Publication: Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 10.1016/j.neuron.2016.11.034. Epub 2016 Dec 22.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hm4Di
• Species: Synthetic
• Promoter: CAG
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Roth lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry