pAAV-FLEX-HM4Di-YFP
(Plasmid
#209193)
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PurposeCre-dependent expression of Hm4Di fused to YFP; Chemogenetics
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 209193 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHm4Di
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SpeciesSynthetic
- Promoter CAG
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRoth lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-FLEX-HM4Di-YFP was a gift from Larry Zweifel (Addgene plasmid # 209193 ; http://n2t.net/addgene:209193 ; RRID:Addgene_209193) -
For your References section:
A Central Amygdala CRF Circuit Facilitates Learning about Weak Threats. Sanford CA, Soden ME, Baird MA, Miller SM, Schulkin J, Palmiter RD, Clark M, Zweifel LS. Neuron. 2017 Jan 4;93(1):164-178. doi: 10.1016/j.neuron.2016.11.034. Epub 2016 Dec 22. 10.1016/j.neuron.2016.11.034 PubMed 28017470
