pENTR4 CDS A. thaliana MAMI
(Plasmid
#209186)
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PurposepENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tags
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR4
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Backbone manufacturerThermo Scientific
- Backbone size w/o insert (bp) 2247
- Total vector size (bp) 3041
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Vector typeGateway-compatible entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAT5G54110.1
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Alt nameATMAMI, MAMI, MEMBRANE-ASSOCIATED MANNITOL-INDUCED
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)794
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Entrez GeneMAMI (a.k.a. AT5G54110, ATMAMI, membrane-associated mannitol-induced)
- Promoter none
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CCCGCCATAAACTGCCAGG
- 3′ sequencing primer CGTTGAATATGGCTCATAACACCC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.09.25.558804v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR4 CDS A. thaliana MAMI was a gift from Lennart Wirthmueller (Addgene plasmid # 209186 ; http://n2t.net/addgene:209186 ; RRID:Addgene_209186) -
For your References section:
Untargeted proteomics identifies plant substrates of the bacterial-derived ADP-ribosyltransferase AvrRpm1. Simranjit Kaur, Thomas Colby, Domenika Thieme, Carsten Proksch, Susanne Matschi, eIvan Matić, and Lennart Wirthmueller. bioRxiv 2023.09.25.558804 10.1101/2023.09.25.558804