pet-MBP
(Plasmid
#209184)
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Purpose(Empty Backbone) Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 209184 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepETARA
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Backbone manufacturerAttila Reményi lab
- Backbone size (bp) 3651
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Modifications to backboneMBP-insert was cloned by XbaI and SpeI
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Vector typeBacterial Expression
- Promoter T7
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Tag
/ Fusion Protein
- HisX6 (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pet-MBP was a gift from Attila Reményi (Addgene plasmid # 209184 ; http://n2t.net/addgene:209184 ; RRID:Addgene_209184) -
For your References section:
Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein-Protein Binding. Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A. Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854. 10.3390/ijms241914854 PubMed 37834301
Map uploaded by the depositor.
