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pcDNA3.1 NKX6-2(1-196)-Venus
(Plasmid #209182)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209182 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NKX6-2 (1-196) fused with Venus
  • Alt name
    GTX
  • Alt name
    NKX6B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1351
  • Entrez Gene
    NKX6-2 (a.k.a. GTX, NKX6.2, NKX6B, SPAX8)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The nucleotide sequence is not the consensus cDNA sequence, since it was optimized for mamalian expression with GeneArt services. However, the amino acid sequence is the same.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 NKX6-2(1-196)-Venus was a gift from Federico Herrera (Addgene plasmid # 209182 ; http://n2t.net/addgene:209182 ; RRID:Addgene_209182)

  • For your References section:

    Is spastic ataxia 8 a protein misfolding disorder?. Ferreira-Peralta P, Franca B, Murtinheira F, Rodrigues MS, Herrera F. Biochim Biophys Acta Mol Basis Dis. 2023 Sep 9;1870(1):166882. doi: 10.1016/j.bbadis.2023.166882. 10.1016/j.bbadis.2023.166882 PubMed 37696463
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