COC053
(Plasmid #209131)

• Purpose: Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.
• Depositing Lab: Christopher A. O'Callaghan
• Publication: O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. doi: 10.1006/abio.1998.2930.

Backbone

• Vector backbone: pGEX-2T
• Total vector size (bp): 5917
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use E. coli strain BL21(DE3)pLysS for protein expression.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Enzyme BirA
• Alt name: birA
• Alt name: DNA-binding transcriptional repressor/biotin-[acetyl-CoA-carboxylase] ligase BirA
• Species: E. coli
• Insert Size (bp): 963
• GenBank ID: NP_418404.1
• Entrez Gene: birA (a.k.a. b3973, ECK3965, bioR, dhbB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GGA GAC AAT GGA TCC AAG GAT AAC ACC GTG CCA CTG AAA TTG
• 3′ sequencing primer: GAT GCC CCA AGC TTG GAT CCT CAT TTT TCT GCA CTA CGC AGG GAT ATT TCA CCG CC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry