FB376
(Plasmid
#209129)
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PurposeMultipartite assembly of the TU for YFP expression with the TU for the auxotrophy marker pyr4 from T. reseei
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209129 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDGB3Ω1
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePgpdA:yfp:TtrpC::Ppyr4:pyr4:Tpyr4
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FB376 was a gift from Jose Marcos (Addgene plasmid # 209129 ; http://n2t.net/addgene:209129 ; RRID:Addgene_209129) -
For your References section:
Agrobacterium tumefaciens-mediated transformation for the genetic modification of the biotechnologically relevant fungus Aspergillus vadensis through synthetic biology. Ropero-Pérez C, Manzanares P, Marcos JF, Garrigues S. Curr Res Biotechnol 2024 10.1016/j.crbiot.2024.100178