pET28a(+)-SUMO-A'-l4-G'
(Plasmid
#209124)
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PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-G'
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a(+)-PT7
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSUMO-A'-l4-G'
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CC: coiled-coil; GEMS: Generalized Extracellular Molecule Sensor. Please visit https://doi.org/10.1101/2023.01.18.524631 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a(+)-SUMO-A'-l4-G' was a gift from Tom de Greef (Addgene plasmid # 209124 ; http://n2t.net/addgene:209124 ; RRID:Addgene_209124) -
For your References section:
Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells. Makri Pistikou AM, Cremers GAO, Nathalia BL, Meuleman TJ, Bogels BWA, Eijkens BV, de Dreu A, Bezembinder MTH, Stassen OMJA, Bouten CCV, Merkx M, Jerala R, de Greef TFA. Nat Commun. 2023 Nov 2;14(1):7001. doi: 10.1038/s41467-023-42810-5. 10.1038/s41467-023-42810-5 PubMed 37919273