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Addgene

pLeo-A-GEMS(JAK-STAT)
(Plasmid #209109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209109 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLeo619-PSV40
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A-GEMS(JAK-STAT)
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Deletion of AA 34-40 and insertion of 34G and 35S downstream of HA tag will not affect plasmid function.

CC: coiled-coil; GEMS: Generalized Extracellular Molecule Sensor. Please visit https://doi.org/10.1101/2023.01.18.524631 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLeo-A-GEMS(JAK-STAT) was a gift from Tom de Greef (Addgene plasmid # 209109 ; http://n2t.net/addgene:209109 ; RRID:Addgene_209109)

  • For your References section:

    Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells. Makri Pistikou AM, Cremers GAO, Nathalia BL, Meuleman TJ, Bogels BWA, Eijkens BV, de Dreu A, Bezembinder MTH, Stassen OMJA, Bouten CCV, Merkx M, Jerala R, de Greef TFA. Nat Commun. 2023 Nov 2;14(1):7001. doi: 10.1038/s41467-023-42810-5. 10.1038/s41467-023-42810-5 PubMed 37919273
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