Combined γB2ΔICD-FRET probe
(Plasmid
#209093)
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PurposemTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCX
- Backbone size w/o insert (bp) 4778
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePcdhgB2
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SpeciesM. musculus (mouse)
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Insert Size (bp)3693
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Entrez GenePcdhgb2
- Promoter Chicken β-actin
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Tag
/ Fusion Protein
- PA tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer CTTCTGGCGTGTGACCGG
- 3′ sequencing primer CCATATGTCCTTCCGAGTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Combined γB2ΔICD-FRET probe was a gift from Takeharu Nagai (Addgene plasmid # 209093 ; http://n2t.net/addgene:209093 ; RRID:Addgene_209093) -
For your References section:
Visualization of trans homophilic interaction of clustered protocadherin in neurons. Hoshino N, Kanadome T, Takasugi T, Itoh M, Kaneko R, Inoue YU, Inoue T, Hirabayashi T, Watanabe M, Matsuda T, Nagai T, Tarusawa E, Yagi T. Proc Natl Acad Sci U S A. 2023 Sep 19;120(38):e2301003120. doi: 10.1073/pnas.2301003120. Epub 2023 Sep 11. 10.1073/pnas.2301003120 PubMed 37695902