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Addgene

pCX:γB2-EC5-VenusΔN3C9-P3
(Plasmid #209092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCX
  • Backbone size w/o insert (bp) 4778
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PcdhgB2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3540
  • Entrez Gene
    Pcdhgb2
  • Promoter Chicken β-actin
  • Tag / Fusion Protein
    • PA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CTTCTGGCGTGTGACCGG
  • 3′ sequencing primer CCATATGTCCTTCCGAGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCX:γB2-EC5-VenusΔN3C9-P3 was a gift from Takeharu Nagai (Addgene plasmid # 209092 ; http://n2t.net/addgene:209092 ; RRID:Addgene_209092)

  • For your References section:

    Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin. Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T. Sci Rep. 2021 Nov 15;11(1):22237. doi: 10.1038/s41598-021-01481-2. 10.1038/s41598-021-01481-2 PubMed 34782670
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