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Addgene

piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122) Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extend ladder and a digestion of the plasmid piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122) with the following enzyme(s): KpnI-HF and PvuI-HF.

Expected Bands:
KpnI-HF: 15,956 bp
PvuI-HF: 11,869 bp and 4087 bp

Results:
We observed the expected results for both digests.