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Addgene

pDonor-PRF1-Exon3
(Plasmid #209075)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209075 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonor
  • Backbone size w/o insert (bp) 3155
  • Total vector size (bp) 6331
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRF1-Exon3 HDRT
  • Alt name
    PRF1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PRF1 (a.k.a. HPLH2, P1, PFP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer gtaaaacgacggccagt
  • 3′ sequencing primer caggaaacagctatgac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonor-PRF1-Exon3 was a gift from Klaus Rajewsky (Addgene plasmid # 209075 ; http://n2t.net/addgene:209075 ; RRID:Addgene_209075)

  • For your References section:

    Precise CRISPR-Cas9 gene repair in autologous memory T cells to treat familial hemophagocytic lymphohistiocytosis. Li X, Wirtz T, Weber T, Lebedin M, Lowenstein ED, Sommermann T, Zach A, Yasuda T, de la Rosa K, Chu VT, Schulte JH, Muller I, Kocks C, Rajewsky K. Sci Immunol. 2024 Feb 2;9(92):eadi0042. doi: 10.1126/sciimmunol.adi0042. Epub 2024 Feb 2. 10.1126/sciimmunol.adi0042 PubMed 38306418
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