pDonor-Prf1-mock-ires-mCherry
(Plasmid
#209074)
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PurposeTargeting vector for the mouse Prf1 locus to correct prf1 gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDonor
- Backbone size w/o insert (bp) 3155
- Total vector size (bp) 6612
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePrf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM)
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Alt nameprf1
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SpeciesM. musculus (mouse)
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Entrez GenePrf1 (a.k.a. Pfn, Pfp, Prf-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer gtaaaacgacggccagt
- 3′ sequencing primer caggaaacagctatgac (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDonor-Prf1-mock-ires-mCherry was a gift from Klaus Rajewsky (Addgene plasmid # 209074 ; http://n2t.net/addgene:209074 ; RRID:Addgene_209074) -
For your References section:
Precise CRISPR-Cas9 gene repair in autologous memory T cells to treat familial hemophagocytic lymphohistiocytosis. Li X, Wirtz T, Weber T, Lebedin M, Lowenstein ED, Sommermann T, Zach A, Yasuda T, de la Rosa K, Chu VT, Schulte JH, Muller I, Kocks C, Rajewsky K. Sci Immunol. 2024 Feb 2;9(92):eadi0042. doi: 10.1126/sciimmunol.adi0042. Epub 2024 Feb 2. 10.1126/sciimmunol.adi0042 PubMed 38306418